WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Polymolecularity and polydispersity in Molecular weight determinations. Pharm Res. 4K, b M.W. Figure5 shows the cumulative amount of the FITC-dextran (M.W. This result could reflect the presence of some aggregated species within the lysozyme sample solution. This method utilizes promising strategies that have made a significant impact in transdermal delivery [6], they include: iontophoresis, microneedles, sonophoresis, radiofrequency (RF), and chemical enhancers [7,8,9,10,11]. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Journal of Applied Polymer Science, 13, 2031-2032. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. De Belder, A. N. (2001). TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. Appl Biochem Biotechnol. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. Masuelli, Martin Alberto (2013). In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Macromolecules, 31, 7712-7716. Biomaterials Research (2003). Molecular weight determination of polysaccharides. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Figure4 shows the histological analysis of micropores in human cadaver skin. Springer Editions. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Morris, G.A. Dependence on Concentration. Corp. (Seoul, Korea). Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. 4, 10, and 20K) was measured using the Transdermal diffusion cell system. Weissella: An Emerging Bacterium with Promising Health Benefits. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Viscosity of Liquids. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The micropores of the human cadaver skin were regenerated according to the time. 4h after microporation, the sizes of the micropore decreased slightly. The genus Weissella: taxonomy, ecology and biotechnological potential. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). Advances in Cosmetic Surgery. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Gekko, K. (1971). The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Progr. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. ; Fran, H.P. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). (2008). The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Google Scholar. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. Format), Citation-(EndNote
The value of qM-H varies from one sample to another one, because it is a function of a and the average molecular weights (Mv, Mw). The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. ; Yevlampieva N.P. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Hydrodynamic radii ( RH) were determined by dynamic light scattering. WebFITC Products. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Banga AK. Carbohydrate Polymers, 39, 377-380. Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Analytical Chemistry. 2016;47:15964. 2. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). AATEX. Sci. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Dextrans can function efficiently as anterograde or retrograde tracers, Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Biotechnology Progress, 19, 544-548. Advanced Drug Delivery Reviews, 60, 1650-1662. Carbohydrate Polymers 72, 356-360. The Mark-Houwink. Journal Non-Newtonian Fluid Mech., 97, 295-301. Recent Pat Drug Deliv Formul. There is a deviation from linearity after 580kDa [69, 70]. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. Walkenhorst, S.; Olivier, R. (2003). In vivo iontophoretic delivery of salmon calcitonin across microporated skin. TEWL measurements were performed before and 30min after microporation. As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth ; Prasad, D.H.L. The particular dextrans which were used initially for conversion into synthetic blood-volume expanders [6, 7], human red blood cells aggregation for increasing the degree of polymerization and hence the molecular weight [8, 9, 10], hydrogels and microspheres [11], in drug transport system and its modifications as nanoparticles [12], in the removal through absorption of contaminant effluents such as heavy metals, organic molecules and inks [13], in the formation of biodegradable films [14, 15]. Colloid Polym. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. Article 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. Chem. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Bohdanecky, Miloslav (1996). 2012;27(3):20212. Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. GPC Symp. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Hrabchak C, Flynn L, Woodhouse KA. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Schematic illustration of transdermal delivery after the application of the microporation device. Each microporated sample was incubated in the skin culture medium. European Biophysical Journal, 36, 85-94. Kumar SV, Tarun P, Kumar TA. Ther. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Doke SK, Dhawale SC. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Front Microbiol. Use of the single point method is correct because it is based in the Huggins method (HM); owing the HM requiring many viscosity and density measurements (at least four) for each Mw, but Solomon Ciuta method with a single measure is sufficient. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. Chemical penetration enhancers for transdermal drug delivery systems. where tr is the column retention time in minutes (2 of 0.9792). At the same temperature, the hydrodynamic radius and the intrinsic viscosity are higher for different molecular weights, while these properties decrease with an increase of temperature at the same molecular weight (Figure 1). Cite this article. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. B 113, 8020-8025. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. (1991). The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. (2013). Determination of intrinsic viscosities of macromolecules and nanoparticles.
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